Publications

Jaenecke J., Bikbaev K., Malagnini M., Bronold J., Yadav S., Apfel UP., Léger C., Birrell JA., Span I., Plumeré N., Winkler M.:
Subunit fusion unlocks rapid in vitro maturation for slowly activating heterodimeric [FeFe]-hydrogenases
In: Chemical Science 17 (2026), p. 7678-7689
ISSN: 2041-6520
DOI: 10.1039/d5sc07299a

Fischer F., Schliehe-Diecks J., Tu JW., Gangnus T., Ho YL., Hebeis M., Alves Avelar LA., Scharov K., Watrin T., Kemkes M., Stachura P., Daugs K., Biermann L., Kremeyer J., Horstick N., Span I., Pandyra AA., Borkhardt A., Gohlke H., Kassack MU., Burckhardt BB., Bhatia S., Kurz T.:
Deciphering the Therapeutic Potential of Novel Pentyloxyamide-Based Class I, IIb HDAC Inhibitors against Therapy-Resistant Leukemia
In: Journal of Medicinal Chemistry (2024)
ISSN: 0022-2623
DOI: 10.1021/acs.jmedchem.4c02024

Martini MA., Bikbaev K., Pang Y., Lorent C., Wiemann C., Breuer N., Zebger I., Debeer S., Span I., Bjornsson R., Birrell JA., Rodriguez-Macia P.:
Binding of exogenous cyanide reveals new active-site states in [FeFe] hydrogenases
In: Chemical Science (2023)
ISSN: 2041-6520
DOI: 10.1039/d2sc06098a
Span I., Etzkorn M.:
RNA-Processing DNAzymes
DOI: 10.1007/978-3-031-36390-0_28

Jansing M., Mielenbrink S., Rosenbach H., Metzger S., Span I.:
Maturation strategy influences expression levels and cofactor occupancy in Fe-S proteins
In: Journal of Biological Inorganic Chemistry (2022)
ISSN: 0949-8257
DOI: 10.1007/s00775-022-01972-1

Borggraefe J., Victor J., Rosenbach H., Viegas A., Gertzen CGW., Wuebben C., Kovacs H., Gopalswamy M., Riesner D., Steger G., Schiemann O., Gohlke H., Span I., Etzkorn M.:
Time-resolved structural analysis of an RNA-cleaving DNA catalyst
In: Nature (2021)
ISSN: 0028-0836
DOI: 10.1038/s41586-021-04225-4
Rosenbach H., Walla E., Cutsail GE., Birrell JA., Pascual-Ortiz M., Debeer S., Fleig U., Span I.:
The Asp1 pyrophosphatase from S. pombe hosts a [2Fe-2S]2+ cluster in vivo
In: Journal of Biological Inorganic Chemistry 26 (2021), p. 93-108
ISSN: 0949-8257
DOI: 10.1007/s00775-020-01840-w

Rodriguez-Macia P., Galle LM., Bjornsson R., Lorent C., Zebger I., Yoda Y., Cramer SP., Debeer S., Span I., Birrell JA.:
Caught in the Hinact: Crystal Structure and Spectroscopy Reveal a Sulfur Bound to the Active Site of an O2-stable State of [FeFe] Hydrogenase
In: Angewandte Chemie International Edition 59 (2020), p. 16786-16794
ISSN: 1433-7851
DOI: 10.1002/anie.202005208
Rosenbach H., Borggraefe J., Victor J., Wuebben C., Schiemann O., Hoyer W., Steger G., Etzkorn M., Span I.:
Influence of monovalent metal ions on metal binding and catalytic activity of the 10–23 DNAzyme
In: Biological Chemistry 402 (2020), p. 99-111
ISSN: 1431-6730
DOI: 10.1515/hsz-2020-0207
Rosenbach H., Victor J., Borggraefe J., Biehl R., Steger G., Etzkorn M., Span I.:
Expanding crystallization tools for nucleic acid complexes using U1A protein variants
In: Journal of Structural Biology 210 (2020), Article No.: 107480
ISSN: 1047-8477
DOI: 10.1016/j.jsb.2020.107480
Rosenbach H., Victor J., Etzkorn M., Steger G., Riesner D., Span I.:
Molecular Features and Metal Ions That Influence 10-23 DNAzyme Activity
In: Molecules 25 (2020), Article No.: 3100
ISSN: 1420-3049
DOI: 10.3390/molecules25133100

Galle LM., Cutsail GE., Nischwitz V., Debeer S., Span I.:
Spectroscopic characterization of the Co-substituted C-terminal domain of rubredoxin-2
In: Biological Chemistry 399 (2018), p. 787-798
ISSN: 1431-6730
DOI: 10.1515/hsz-2018-0142
Pascual-Ortiz M., Saiardi A., Walla E., Jakopec V., Kuenzel NA., Span I., Vangala A., Fleig U.:
Asp1 bifunctional activity modulates spindle function via controlling cellular inositol pyrophosphate levels in Schizosaccharomyces pombe
In: Molecular and Cellular Biology 38 (2018), Article No.: e00047-18
ISSN: 0270-7306
DOI: 10.1128/MCB.00047-18

Trana EN., Nocek JM., Vander Woude J., Span I., Smith SM., Rosenzweig AC., Hoffman BM.:
Charge-Disproportionation Symmetry Breaking Creates a Heterodimeric Myoglobin Complex with Enhanced Affinity and Rapid Intracomplex Electron Transfer
In: Journal of the American Chemical Society 138 (2016), p. 12615-12628
ISSN: 1520-5126
DOI: 10.1021/jacs.6b07672

Kathman SG., Span I., Smith AT., Xu Z., Zhan J., Rosenzweig AC., Statsyuk AV.:
A Small Molecule That Switches a Ubiquitin Ligase from a Processive to a Distributive Enzymatic Mechanism
In: Journal of the American Chemical Society 137 (2015), p. 12442-12445
ISSN: 1520-5126
DOI: 10.1021/jacs.5b06839

Span I., Wang K., Eisenreich W., Bacher A., Zhang Y., Oldfield E., Groll M.:
Insights into the binding of pyridines to the iron-sulfur enzyme IspH
In: Journal of the American Chemical Society 136 (2014), p. 7926-7932
ISSN: 1520-5126
DOI: 10.1021/ja501127j

Span I., Wang K., Wang W., Jauch J., Eisenreich W., Bacher A., Oldfield E., Groll M.:
Structures of fluoro, amino, and thiol inhibitors bound to the [Fe 4S4] protein IspH
In: Angewandte Chemie International Edition 52 (2013), p. 2118-2121
ISSN: 1433-7851
DOI: 10.1002/anie.201208469

Span I., Graewert T., Bacher A., Eisenreich W., Groll M.:
Crystal structures of mutant IspH proteins reveal a rotation of the substrate's hydroxymethyl group during catalysis
In: Journal of Molecular Biology 416 (2012), p. 1-9
ISSN: 0022-2836
DOI: 10.1016/j.jmb.2011.11.033
Span I., Wang K., Wang W., Zhang Y., Bacher A., Eisenreich W., Li K., Schulz C., Oldfield E., Groll M.:
Discovery of acetylene hydratase activity of the iron-sulphur protein IspH
In: Nature Communications 3 (2012), Article No.: 1042
ISSN: 2041-1723
DOI: 10.1038/ncomms2052
Wang W., Wang K., Span I., Jauch J., Bacher A., Groll M., Oldfield E.:
Are free radicals involved in IspH catalysis? An EPR and crystallographic investigation
In: Journal of the American Chemical Society 134 (2012), p. 11225-11234
ISSN: 1520-5126
DOI: 10.1021/ja303445z

Graewert T., Span I., Bacher A., Groll M.:
Reductive dehydroxylation of allyl alcohols by IspH protein
In: Angewandte Chemie International Edition 49 (2010), p. 8802-8809
ISSN: 1433-7851
DOI: 10.1002/anie.201000833
Graewert T., Span I., Eisenreich W., Rohdich F., Eppinger J., Bacher A., Groll M.:
Probing the reaction mechanism of IspH protein by x-ray structure analysis
In: Proceedings of the National Academy of Sciences of the United States of America 107 (2010), p. 1077-1081
ISSN: 0027-8424
DOI: 10.1073/pnas.0913045107