Methods and instruments
Methods and instruments
The research group has access to a wide array of techniques for sample preparation and clean-up. Particularly strategies on liquid-liquid extraction and fractionation using solid phase extraction are employed.
For the analysis of target molecules a range of chromatographic separation and detection techniques are available within the group and through collaborations. Examples can be seen below:
Gas chromatography coupled to flame ionisation detection (GC-FID)

HP 5890 series II plus gas chromatograph with flame ionisation detector
- Split/Splitless injector and Cold Injection System (Gerstel)
- Fully programmable CTC CombiPAL autosampler system with tray for 96 samples
Applications: Workhorse in the laboratory, used for screening of samples and quantification of low molecular weight lipids
Gas chromatography coupled to mass spectrometry (GC-MS)

Trace GC Ultra
- Split/Splitless
- injector AS 3000 autosampler for 120 samples
DSQ II mass spectrometer
- single quadrupole instrument
- mass range of m/z 50-1050
- quadrupole scan rate of > 10 000 amu/s
- separate and simultaneous acquisition of full scan and SIM data
- Option for chemical ionisation
Applications: Analysis of small molecules and simple lipids such as fatty acids or sterols
More instruments will be added soon!