The food chemistry research group at FAU focuses on the molecular determinants of food bioactivity and functionality. Foods are complex systems, which are composed of thousands of different components. In order to understand how the foods’ molecular composition modulates their (bio-)function, comprehensive profiling methods are applied based on cutting-edge mass spectrometry methods and bioinformatic tools.
Food peptides strongly influence the quality and possible health benefits of food. The combination of different high-resolution mass spectrometry methods allows for comprehensive qualitative and quantitative peptide profiling. Linked to virtual screening and bioinformatics analysis, the peptide profiles lead to the identification of novel functional ingredients, such as, e.g., highly active antimicrobial peptides from milk and plant sources.
Peptide profiling in combination with bioinformatics analysis can also identify marker compounds to control the authenticity of food, for example of milk and milk products, or to detect food fraud. Since various external and internal parameters influence the milk peptidome, a peptide marker set reflects various food properties, such as species, fermentation, storage or processing conditions.
High-resolution mass spectrometry linked to bioinformatics tools allows comprehensive profiling of the protein composition in foods. Thus, the qualitative protein composition reveals bioactive, techno-functional proteins and detects molecular differences between samples. Quantitative proteome analysis is a highly flexible method for the absolute and relative quantification of protein profiles in a complex food. Qualitative and quantitative protein profiles decipher the molecular determinants of food quality, health benefits and safety with the goal to control and rationally design its properties.
On the protein level, food proteomics aims for the comprehensive analysis of individual protein structures. Our work group develops and applies mass spectrometry methods for the systematic survey of non-enzymatic posttranslational modifications including structure elucidation and the time-dependent quantification of each specific product and binding site. The most prevalent non-enzymatic posttranslational modifications are oxidation, glycation to advanced glycation end-products (AGE), and Maillard reactions, which are formed, for example, during food processing.
Bioactive molecules interact with various cellular targets. Cellular proteomics aims for the comprehensive description of cellular reactions towards bioactive molecules by their time-dependent protein expression profile. The profile eventually allows the precise prediction of the tested bioactive components. Our work group developed, for example, pluridimensional targeted proteome assays to evaluate cytoprotective food components.
Within the DFG Research Training Group “Medicinal Chemistry of Selective GPCR Ligands” (RTG1910), we develop multiplexed targeted proteome assays to monitor downstream signaling events resulting from functionally selective ligand binding. Untargeted profiling of protein expression can identify novel signaling events. The objective is to develop a fast and flexible assay system based on proteomics tools to address ligand-biased signaling of ligands of G protein-coupled receptors.
As a part of the International Graduate Programme “Receptor Dynamics: Emerging Paradigms for Novel Drugs” of Elite Network of Bavaria, our group addresses the ligand-specific conformation of G protein-coupled receptors by a quantitative mass spectrometry approach.
“Neurotrition” describes the interaction between nutrition and neuronal function. Nutrients and diet can modulate brain functionality and brain activity, while the brain’s activity patterns in turn influence the nutritional intake.
The ongoing projects aim to elucidate how molecularly defined food components affect the brain functionality, and how the neurophysiological processes influence the type and quantity of consumed food.
The research unit Food Safety and Quality is a scientific platform for interdisciplinary research projects focusing on the quality enhancement of food and the improvement of food safety. For this purpose, different disciplines at Friedrich-Alexander-Universität Erlangen-Nürnberg pool cutting-edge research in food-related projects. Translational projects transfer the results at an early stage to technical applications.
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- Gensberger-Reigl, Sabrina, and Andreas Konopa. "Optimization of Cleaning Processes based on the quantitative Analysis of Chlorate." Deutsche Lebensmittel-Rundschau 115.10 (2019): 444-450.
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- Schulz, Carolin, Chafia Chiheb, and Monika Pischetsrieder. "Quantification of co-, n-, and ad-lupulone in hop-based dietary supplements and phytopharmaceuticals and modulation of their contents by the extraction method." Journal of Pharmaceutical and Biomedical Analysis 168 (2019): 124-132.
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- Östreicher, Christiane, Sabrina Gensberger-Reigl, and Monika Pischetsrieder. "Targeted mass spectrometry to monitor nuclear accumulation of endogenous Nrf2 and its application to SH-SY5Y cells stimulated with food components." Analytical and Bioanalytical Chemistry 411.6 (2019): 1273-1286.
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- Liu, Yufang, and Monika Pischetsrieder. "Absolute quantification of two antimicrobial peptides alpha(S2)-casein(182-207) and alpha(S2)-casein(151-181) in bovine milk by UHPLC-ESI-MS/MS in sMRM mode." Food Chemistry 261 (2018): 15-20.
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- Pignitter, Marc, et al. "Regioisomeric distribution of 9-and 13-hydroperoxy linoleic acid in vegetable oils during storage and heating." Journal of the Science of Food and Agriculture 98.3 (2018): 1240-1247.
- Pischetsrieder, Monika. "Global Food-Related Challenges: What Chemistry Has Achieved and What Remains to Be Done." Angewandte Chemie International Edition 57.36 (2018): 11476-11477.
- Schulz, Carolin, et al. "Activation of membrane-located Ca2+ channels by hop beta acids and their tricyclic transformation products." Food Chemistry 252 (2018): 215-227.
- Yu, Yidong, et al. "Mating-type factor-specific regulation of the fumagillin/pseurotin secondary metabolite supercluster in Aspergillus fumigatus." Molecular Microbiology 110.6 (2018): 1045-1065.
- Bartenbacher, Sven, Christiane Östreicher, and Monika Pischetsrieder. "Profiling of antioxidative enzyme expression induced by various food components using targeted proteome analysis." Molecular nutrition & food research 61.9 (2017).
- Dalabasmaz, Sevim, Jennifer Ebner, and Monika Pischetsrieder. "Identification of the Peptide PyroQ-beta Casein(194-209) as a Highly Specific and Sensitive Marker to Differentiate between Ultrahigh-Temperature Processed (UHT) Milk and Mildly Heated Milk." Journal of Agricultural and Food Chemistry 65.49 (2017): 10781-10791.
- Emirbayer, Pelin Esma, et al. "Targeted label-free quantification of interleukin-8 in PMA-activated U937 cell secretome by nanoLC-ESI-MS/MS-sSRM." Proteomics (2017).
- Emirbayer, Pelin Esma, et al. "Proteomic Response of Human Umbilical Vein Endothelial Cells to Histamine Stimulation." Proteomics 17.21 (2017).
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- Li, Xuan, Linwei Liu, and Monika Pischetsrieder. "Pomegranate (Punica granatum L,) wine polyphenols affect Nrf2 activation and antioxidant enzyme expression in human neuroblastoma cells (SH-SY5Y)." Journal of Functional Foods 38 (2017): 140-150.
- Liu, Yufang, and Monika Pischetsrieder. "Identification and Relative Quantification of Bioactive Peptides Sequentially Released during Simulated Gastrointestinal Digestion of Commercial Kefir." Journal of agricultural and food chemistry 65.9 (2017): 1865-1873.
- Oßmann, Barbara, et al. "Development of an optimal filter substrate for the identification of small microplastic particles in food by micro-Raman spectroscopy." Analytical and Bioanalytical Chemistry 409.16 (2017): 4099-4109.
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- Vetter, Walter, et al. "The use of countercurrent chromatography in the separation of nonpolar lipid compounds." Journal of Chromatography A 1501 (2017): 51-60.
- Östreicher, Christiane, Sven Bartenbacher, and Monika Pischetsrieder. "Targeted proteome analysis with isotope-coded protein labels for monitoring the influence of dietary phytochemicals on the expression of cytoprotective proteins in primary human colon cells." Journal of Proteomics 166 (2017): 27-38.